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cdna ha pkc α  (Addgene inc)


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    Structured Review

    Addgene inc cdna ha pkc α
    (A) GNAQQ209L transfected alone or combined with <t>PKC</t> δ or ε into 293FT cells increased Ras-GTP levels. 293FT cells were transfected with indicated <t>cDNA</t> plasmids for 24 hr. Ras-GTP pull-down was performed and detected by western blot with a pan-Ras antibody. TPA stimulation was used a positive control. Expression levels of GNAQQ209L were monitored with Glu-Glu (EE) tag. PKC δ and ε were detected via HA tags.
    Cdna Ha Pkc α, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+ha+pkc+%CE%B1/PKC+alpha+CAT+(Plasmid+%2321234)/pmc05499527-106-0-6
    Average 90 stars, based on 16 article reviews
    cdna ha pkc α - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma"

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma

    Journal: Cancer cell

    doi: 10.1016/j.ccell.2017.04.002

    (A) GNAQQ209L transfected alone or combined with PKC δ or ε into 293FT cells increased Ras-GTP levels. 293FT cells were transfected with indicated cDNA plasmids for 24 hr. Ras-GTP pull-down was performed and detected by western blot with a pan-Ras antibody. TPA stimulation was used a positive control. Expression levels of GNAQQ209L were monitored with Glu-Glu (EE) tag. PKC δ and ε were detected via HA tags.
    Figure Legend Snippet: (A) GNAQQ209L transfected alone or combined with PKC δ or ε into 293FT cells increased Ras-GTP levels. 293FT cells were transfected with indicated cDNA plasmids for 24 hr. Ras-GTP pull-down was performed and detected by western blot with a pan-Ras antibody. TPA stimulation was used a positive control. Expression levels of GNAQQ209L were monitored with Glu-Glu (EE) tag. PKC δ and ε were detected via HA tags.

    Techniques Used: Transfection, Western Blot, Positive Control, Expressing

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Activation Assay, Microarray, Fractionation, Expressing, Cell Culture

    Related Articles

    Transfection:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Western Blot:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Positive Control:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Expressing:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Recombinant:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Activation Assay:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Microarray:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Fractionation:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Cell Culture:

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma
    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.



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    cdna ha pkc α  (Addgene inc)
    90
    Addgene inc cdna ha pkc α
    (A) GNAQQ209L transfected alone or combined with <t>PKC</t> δ or ε into 293FT cells increased Ras-GTP levels. 293FT cells were transfected with indicated <t>cDNA</t> plasmids for 24 hr. Ras-GTP pull-down was performed and detected by western blot with a pan-Ras antibody. TPA stimulation was used a positive control. Expression levels of GNAQQ209L were monitored with Glu-Glu (EE) tag. PKC δ and ε were detected via HA tags.
    Cdna Ha Pkc α, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+ha+pkc+%CE%B1/PKC+alpha+CAT+(Plasmid+%2321234)/pmc05499527-106-0-6
    Average 90 stars, based on 1 article reviews
    cdna ha pkc α - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) GNAQQ209L transfected alone or combined with PKC δ or ε into 293FT cells increased Ras-GTP levels. 293FT cells were transfected with indicated cDNA plasmids for 24 hr. Ras-GTP pull-down was performed and detected by western blot with a pan-Ras antibody. TPA stimulation was used a positive control. Expression levels of GNAQQ209L were monitored with Glu-Glu (EE) tag. PKC δ and ε were detected via HA tags.

    Journal: Cancer cell

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma

    doi: 10.1016/j.ccell.2017.04.002

    Figure Lengend Snippet: (A) GNAQQ209L transfected alone or combined with PKC δ or ε into 293FT cells increased Ras-GTP levels. 293FT cells were transfected with indicated cDNA plasmids for 24 hr. Ras-GTP pull-down was performed and detected by western blot with a pan-Ras antibody. TPA stimulation was used a positive control. Expression levels of GNAQQ209L were monitored with Glu-Glu (EE) tag. PKC δ and ε were detected via HA tags.

    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Techniques: Transfection, Western Blot, Positive Control, Expressing

    KEY RESOURCES TABLE

    Journal: Cancer cell

    Article Title: RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma

    doi: 10.1016/j.ccell.2017.04.002

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: cDNA: HA-PKC α (catalytic domain) , addgene , 21234.

    Techniques: Recombinant, Activation Assay, Microarray, Fractionation, Expressing, Cell Culture